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STEMCELL Technologies Inc easysep human memory cd4+ t cell enrichment kit
Easysep Human Memory Cd4+ T Cell Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easysep+human+memory+cd4+++t+cell+enrichment+kit/easysep+human+t+cell+isolation+kit/pm40333189-803-21-38
Average 90 stars, based on 1 article reviews
easysep human memory cd4+ t cell enrichment kit - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Isolation:

Article Title: Estrogen receptor-1 is a key regulator of HIV-1 latency that imparts gender-specific restrictions on the latent reservoir.
Article Snippet: .. CD4+ memory cells were negatively isolated using robotic magnetic bead isolation using the EasySep Human Memory CD4 T Cell Enrichment Kit (19157; STEMCELL Technologies). ..

Article Title: Estrogen receptor-1 is a key regulator of HIV-1 latency that imparts gender-specific restrictions on the latent reservoir
Article Snippet: .. CD4 + memory cells were negatively isolated using robotic magnetic bead isolation using the EasySep Human Memory CD4 T Cell Enrichment Kit (19157; STEMCELL Technologies). ..

Article Title: Inhibition of HIV Replication by Apolipoprotein A-I Binding Protein Targeting the Lipid Rafts
Article Snippet: .. Memory CD4 T-cells were isolated from PBMCs (Peripheral Blood Leuko Pak; AllCells) by negative selection (EasySep human memory CD4 T cell enrichment kit; Stemcell Technologies) following the manufacturer’s instructions. .. Isolated memory CD4 T-cells were then engrafted into 7-to-9-week-old NOD.Cg-Prkdcscid Il2rgtm1wjl/SzJ (NSG) mice (The Jackson Laboratory) at 107 cells per animal via tail vein injection.

Gradient Centrifugation:

Article Title: Human Monocytes Promote Th1 and Th17 Responses to Streptococcus pneumoniae
Article Snippet: Monocytes and CD4 T cells were purified by negative selection with RosetteSep human monocyte enrichment cocktail and RosetteSep human CD4 T cell enrichment cocktail (StemCell Technologies). .. CD4 memory T cells were prepared with an EasySep human memory CD4 T cell enrichment kit (StemCell Technologies) after a standard density gradient centrifugation with Ficoll-Paque Plus (GE Healthcare). .. Monocytes were resuspended in RPMI 1640 (Invitrogen), and CD4 T cells were resuspended in Iscove’s modified Dulbecco’s medium (IMDM) (Sigma-Aldrich); both cell culture media were supplemented with 10% heat-inactivated fetal calf serum (FCS) and 2 mM L-glutamine (Invitrogen).

Activation Assay:

Article Title: Peripheral T Follicular Helper Cells Are the Major HIV Reservoir within Central Memory CD4 T Cells in Peripheral Blood from Chronically HIV-Infected Individuals on Combination Antiretroviral Therapy
Article Snippet: .. To avoid nonspecific cellular activation, fresh PBMC or cryopreserved PBMC that had been rested for 4 h after thawing were processed to isolate memory CD4 T cells (CD45RA − ) by negative selection with the EasySep human memory CD4 T cell enrichment kit (Stemcell Technologies) according to the manufacturer's protocol and sorted on a BD FACS Aria to obtain pTfh and non-pTfh cell subsets at purities of ≥99%. ..

Selection:

Article Title: Peripheral T Follicular Helper Cells Are the Major HIV Reservoir within Central Memory CD4 T Cells in Peripheral Blood from Chronically HIV-Infected Individuals on Combination Antiretroviral Therapy
Article Snippet: .. To avoid nonspecific cellular activation, fresh PBMC or cryopreserved PBMC that had been rested for 4 h after thawing were processed to isolate memory CD4 T cells (CD45RA − ) by negative selection with the EasySep human memory CD4 T cell enrichment kit (Stemcell Technologies) according to the manufacturer's protocol and sorted on a BD FACS Aria to obtain pTfh and non-pTfh cell subsets at purities of ≥99%. ..

Article Title: Inhibition of HIV Replication by Apolipoprotein A-I Binding Protein Targeting the Lipid Rafts
Article Snippet: .. Memory CD4 T-cells were isolated from PBMCs (Peripheral Blood Leuko Pak; AllCells) by negative selection (EasySep human memory CD4 T cell enrichment kit; Stemcell Technologies) following the manufacturer’s instructions. .. Isolated memory CD4 T-cells were then engrafted into 7-to-9-week-old NOD.Cg-Prkdcscid Il2rgtm1wjl/SzJ (NSG) mice (The Jackson Laboratory) at 107 cells per animal via tail vein injection.

FACS:

Article Title: Peripheral T Follicular Helper Cells Are the Major HIV Reservoir within Central Memory CD4 T Cells in Peripheral Blood from Chronically HIV-Infected Individuals on Combination Antiretroviral Therapy
Article Snippet: .. To avoid nonspecific cellular activation, fresh PBMC or cryopreserved PBMC that had been rested for 4 h after thawing were processed to isolate memory CD4 T cells (CD45RA − ) by negative selection with the EasySep human memory CD4 T cell enrichment kit (Stemcell Technologies) according to the manufacturer's protocol and sorted on a BD FACS Aria to obtain pTfh and non-pTfh cell subsets at purities of ≥99%. ..



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